The technical and resource advantages of Zo determine the outstanding service quality of Zoonbio. We constructed the characteristic plasmids with His tag and Sumo tag. At the same time, we screened Arctic ExpressTM high-efficient expression strains of Low-Temperature Induction system, which can induce expression at 11 ℃ and ultra-low temperature. The soluble expression probability of recombinant protein supernatant was greatly increased, and the expression success rate and the original were increased. The activity probability of the expressed products.
Optimization of expression conditions | ||
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Screening and optimization of strains | Temperature gradient optimization | Vector screening optimization |
M:protein M -:uninduced +:induce ↑:supernatant ↓:precipitation |
Identification of protein expression | |
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70KD protein expression | 30KD protein expression |
M:Protein Marker 1:unconditioned control 2:post-induction bacteria(clones 1) 3:post-induction bacteria(clones 2) 4:expression supernatant 5:expression precipitation |
Protein expression and purification | |
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85% purity | 90% purity |
prokaryotic Purification Map: M:marker 1:samples after crushing 2:flow out 3:eluent |
Related services:
mammalian transient protein expression service
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