Summary

The technical and resource advantages of Zo determine the outstanding service quality of Zoonbio. We constructed the characteristic plasmids with His tag and Sumo tag. At the same time, we screened Arctic ExpressTM high-efficient expression strains of Low-Temperature Induction system, which can induce expression at 11 ℃ and ultra-low temperature. The soluble expression probability of recombinant protein supernatant was greatly increased, and the expression success rate and the original were increased. The activity probability of the expressed products.

Characteristic Vector

case

Optimization of expression conditions

case case case
Screening and optimization of strains Temperature gradient optimization      Vector screening optimization

M:protein M    -:uninduced    +:induce     ↑:supernatant    ↓:precipitation



Identification of protein expression

case case
    70KD protein expression     30KD protein expression

M:Protein Marker    1:unconditioned control    2:post-induction bacteria(clones 1)    
3:post-induction bacteria(clones 2)    4:expression supernatant    5:expression precipitation



Protein expression and purification

case case
      85% purity        90% purity

prokaryotic Purification Map:    M:marker    1:samples after crushing     2:flow out    3:eluent

Related services:

mammalian transient protein expression service

baculovirus-insect cell protein expression service

pichia pastoris protein expression service